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bv2 microglia cl 0493  (Procell Inc)

 
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    Procell Inc bv2 microglia cl 0493
    Preparation and characterization of 3D-ABs and Mxene nanosheets. (A) Graphical illustration of the 3D-ABs isolation procedure. (B) 2D-BMSCs and 3D-BMSCs stained by CD29 and CD44 (scale bar, 40 μm). (C) SEM images of 3D-ABs (scale bar, 1 μm). (D) 3D-ABs stained by CIQC (scale bar, 10 μm). (E) DLS analysis for the 3D-ABs. (F) Expression of biomarkers of 3D-ABs and Hydrogel-3D-ABs including C1QC, C3B, H2B, and H3, β-actin was utilized as a loading control. (G) FCM analysis of the percentage of Dil-positive PC12 and <t>BV2</t> cells after treatment with DiI-labelled 3D-ABs. (H) Uptake of Dil-labelled 3D-ABs and Hydrogel-3D-ABs by PC12 and BV2 cells (scale bar, 20 μm). (I) Frozen sections of DiI-labelled 3D-ABs and Hydrogel-3D-ABs treated spinal cord were stained for Neun and CD68 (scale bars, 20 μm). (J) SEM and EDS elemental mapping images of the Mxene nanosheets (scale bar, 20 μm). (K) Live/dead cell staining images for PC12 and BV2 cells after different treatments (scale bar, 200 μm).
    Bv2 Microglia Cl 0493, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bv2+microglia+cl+0493/pmc12907102-326-0-6?v=Procell+Inc
    Average 86 stars, based on 1 article reviews
    bv2 microglia cl 0493 - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis"

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.01.043

    Preparation and characterization of 3D-ABs and Mxene nanosheets. (A) Graphical illustration of the 3D-ABs isolation procedure. (B) 2D-BMSCs and 3D-BMSCs stained by CD29 and CD44 (scale bar, 40 μm). (C) SEM images of 3D-ABs (scale bar, 1 μm). (D) 3D-ABs stained by CIQC (scale bar, 10 μm). (E) DLS analysis for the 3D-ABs. (F) Expression of biomarkers of 3D-ABs and Hydrogel-3D-ABs including C1QC, C3B, H2B, and H3, β-actin was utilized as a loading control. (G) FCM analysis of the percentage of Dil-positive PC12 and BV2 cells after treatment with DiI-labelled 3D-ABs. (H) Uptake of Dil-labelled 3D-ABs and Hydrogel-3D-ABs by PC12 and BV2 cells (scale bar, 20 μm). (I) Frozen sections of DiI-labelled 3D-ABs and Hydrogel-3D-ABs treated spinal cord were stained for Neun and CD68 (scale bars, 20 μm). (J) SEM and EDS elemental mapping images of the Mxene nanosheets (scale bar, 20 μm). (K) Live/dead cell staining images for PC12 and BV2 cells after different treatments (scale bar, 200 μm).
    Figure Legend Snippet: Preparation and characterization of 3D-ABs and Mxene nanosheets. (A) Graphical illustration of the 3D-ABs isolation procedure. (B) 2D-BMSCs and 3D-BMSCs stained by CD29 and CD44 (scale bar, 40 μm). (C) SEM images of 3D-ABs (scale bar, 1 μm). (D) 3D-ABs stained by CIQC (scale bar, 10 μm). (E) DLS analysis for the 3D-ABs. (F) Expression of biomarkers of 3D-ABs and Hydrogel-3D-ABs including C1QC, C3B, H2B, and H3, β-actin was utilized as a loading control. (G) FCM analysis of the percentage of Dil-positive PC12 and BV2 cells after treatment with DiI-labelled 3D-ABs. (H) Uptake of Dil-labelled 3D-ABs and Hydrogel-3D-ABs by PC12 and BV2 cells (scale bar, 20 μm). (I) Frozen sections of DiI-labelled 3D-ABs and Hydrogel-3D-ABs treated spinal cord were stained for Neun and CD68 (scale bars, 20 μm). (J) SEM and EDS elemental mapping images of the Mxene nanosheets (scale bar, 20 μm). (K) Live/dead cell staining images for PC12 and BV2 cells after different treatments (scale bar, 200 μm).

    Techniques Used: Isolation, Staining, Expressing, Control

    Composite hydrogel inhibits oxidative damage in vitro and in vivo. (A) DCFH-DA staining of PC12 and BV2 cells treated with different hydrogel under TBHP treatment (scale bar, 200 μm). (B) Frozen sections of different hydrogel-treated spinal cords were stained for DHE (scale bar: 50 μm). (C, D) DCFH-DA integrated intensity analysis of PC12 and BV2 cells (n = 3). (E) Analysis and quantification of DHE integrated intensity in each group (n = 3). (F) Transcriptome GSEA analysis. The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.
    Figure Legend Snippet: Composite hydrogel inhibits oxidative damage in vitro and in vivo. (A) DCFH-DA staining of PC12 and BV2 cells treated with different hydrogel under TBHP treatment (scale bar, 200 μm). (B) Frozen sections of different hydrogel-treated spinal cords were stained for DHE (scale bar: 50 μm). (C, D) DCFH-DA integrated intensity analysis of PC12 and BV2 cells (n = 3). (E) Analysis and quantification of DHE integrated intensity in each group (n = 3). (F) Transcriptome GSEA analysis. The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Techniques Used: In Vitro, In Vivo, Staining, Comparison

    Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.
    Figure Legend Snippet: Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Techniques Used: In Vitro, Western Blot, Expressing, Control, Immunofluorescence, Fluorescence, Staining, Comparison



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    Image Search Results


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    Journal: Bioactive Materials

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

    doi: 10.1016/j.bioactmat.2026.01.043

    Figure Lengend Snippet: Preparation and characterization of 3D-ABs and Mxene nanosheets. (A) Graphical illustration of the 3D-ABs isolation procedure. (B) 2D-BMSCs and 3D-BMSCs stained by CD29 and CD44 (scale bar, 40 μm). (C) SEM images of 3D-ABs (scale bar, 1 μm). (D) 3D-ABs stained by CIQC (scale bar, 10 μm). (E) DLS analysis for the 3D-ABs. (F) Expression of biomarkers of 3D-ABs and Hydrogel-3D-ABs including C1QC, C3B, H2B, and H3, β-actin was utilized as a loading control. (G) FCM analysis of the percentage of Dil-positive PC12 and BV2 cells after treatment with DiI-labelled 3D-ABs. (H) Uptake of Dil-labelled 3D-ABs and Hydrogel-3D-ABs by PC12 and BV2 cells (scale bar, 20 μm). (I) Frozen sections of DiI-labelled 3D-ABs and Hydrogel-3D-ABs treated spinal cord were stained for Neun and CD68 (scale bars, 20 μm). (J) SEM and EDS elemental mapping images of the Mxene nanosheets (scale bar, 20 μm). (K) Live/dead cell staining images for PC12 and BV2 cells after different treatments (scale bar, 200 μm).

    Article Snippet: BV2 microglia (CL-0493) were obtained from Procell Life Science & Technology Co., Ltd.

    Techniques: Isolation, Staining, Expressing, Control

    Composite hydrogel inhibits oxidative damage in vitro and in vivo. (A) DCFH-DA staining of PC12 and BV2 cells treated with different hydrogel under TBHP treatment (scale bar, 200 μm). (B) Frozen sections of different hydrogel-treated spinal cords were stained for DHE (scale bar: 50 μm). (C, D) DCFH-DA integrated intensity analysis of PC12 and BV2 cells (n = 3). (E) Analysis and quantification of DHE integrated intensity in each group (n = 3). (F) Transcriptome GSEA analysis. The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Journal: Bioactive Materials

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

    doi: 10.1016/j.bioactmat.2026.01.043

    Figure Lengend Snippet: Composite hydrogel inhibits oxidative damage in vitro and in vivo. (A) DCFH-DA staining of PC12 and BV2 cells treated with different hydrogel under TBHP treatment (scale bar, 200 μm). (B) Frozen sections of different hydrogel-treated spinal cords were stained for DHE (scale bar: 50 μm). (C, D) DCFH-DA integrated intensity analysis of PC12 and BV2 cells (n = 3). (E) Analysis and quantification of DHE integrated intensity in each group (n = 3). (F) Transcriptome GSEA analysis. The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Article Snippet: BV2 microglia (CL-0493) were obtained from Procell Life Science & Technology Co., Ltd.

    Techniques: In Vitro, In Vivo, Staining, Comparison

    Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Journal: Bioactive Materials

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

    doi: 10.1016/j.bioactmat.2026.01.043

    Figure Lengend Snippet: Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Article Snippet: BV2 microglia (CL-0493) were obtained from Procell Life Science & Technology Co., Ltd.

    Techniques: In Vitro, Western Blot, Expressing, Control, Immunofluorescence, Fluorescence, Staining, Comparison